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wnt2  (Proteintech)


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    Proteintech wnt2
    Wnt2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wnt2/product/Proteintech
    Average 94 stars, based on 26 article reviews
    wnt2 - by Bioz Stars, 2026-02
    94/100 stars

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    Effect of LA on Wnt/β‐catenin pathway in MCF‐7 cells. (A) The migration of cells treated with LA into the wound was visualized at 0, 6, 12, and 24 h under an inverted microscope, and the percentage of wound closure was evaluated statistically. (B–I) Expressions of WNT2 , DVL1 , AXIN1 , β‐Catenin , TCF‐4 , CCND1 , c‐MYC , and CDK1 genes analyzed by qPCR in LA‐treated MCF‐7 cells at 48 h. (J–M) Protein expressions of WNT2, GSK3‐ β, and β‐catenin determined by Western blot analysis. The experiment was performed in three biological and technical replicates. * p < 0.05, ** p < 0.01, and *** p < 0.001 in relation to the control. Scale bar, 500 μm.

    Journal: Pharmacology Research & Perspectives

    Article Title: Lobaric Acid Exhibits Anticancer Potential by Modulating the Wnt/β‐Catenin Signaling Pathway in MCF‐7 Cells

    doi: 10.1002/prp2.70142

    Figure Lengend Snippet: Effect of LA on Wnt/β‐catenin pathway in MCF‐7 cells. (A) The migration of cells treated with LA into the wound was visualized at 0, 6, 12, and 24 h under an inverted microscope, and the percentage of wound closure was evaluated statistically. (B–I) Expressions of WNT2 , DVL1 , AXIN1 , β‐Catenin , TCF‐4 , CCND1 , c‐MYC , and CDK1 genes analyzed by qPCR in LA‐treated MCF‐7 cells at 48 h. (J–M) Protein expressions of WNT2, GSK3‐ β, and β‐catenin determined by Western blot analysis. The experiment was performed in three biological and technical replicates. * p < 0.05, ** p < 0.01, and *** p < 0.001 in relation to the control. Scale bar, 500 μm.

    Article Snippet: Subsequently, membrane incubation was performed overnight at 4°C using P53 antibody (Proteintech, 10442‐1‐AP, 1:1000), BCL2 antibody (Proteintech 12789‐1‐AP 1:500), WNT2 antibody (Proteintech, 66656‐I‐Ig, 1:1000), AXIN antibody (Proteintech, 68093‐1‐Ig, 1:1000), β‐Catenin antibody (Santa Cruz Biotechnology, sc‐7963, 1:1000), GSK3‐ β antibody (Santa Cruz, sc‐377213, 1:1000), and β‐Actin antibody (Santa Cruz Biotechnology, sc‐47778, 1:1000).

    Techniques: Migration, Inverted Microscopy, Western Blot, Control